Human Brain Microvascular Endothelial Cells (HBMVEC)

Product Description

Human Brain Microvascular Endothelial Cells (HBMVEC) are the major element of the blood-brain barrier that shields the brain against toxins and immune cells via paracellular, transcellular, transporter, and extracellular matrix proteins ^[1]. HBMVEC are morphologically different from the peripheral endothelium. Brain endothelial cells lack fenestrations, have minimal pinocytic activity, are connected by tight junctions and have a large number of mitochondria associated with high metabolic activity ^[2]. Like peripheral endothelial cells, however, HBMVEC express, or can be induced to express, cell adhesion molecules on their surface that regulate the extravasation of leukocytes into the brain. HBMVEC have been widely used for studying the molecular and cellular function of blood-brain barrier ^[3].

iXCells Biotechnologies provides high quality HBMVEC, which are isolated from human brain and cryopreserved at P2, with ≥ 0.5 million cells in each vial. These HBMVEC express von Willebrand Factor (vWF), CD31 (PECAM), and Dil-Ac-LDL by uptake. They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can be further expanded for no more than 3 passages in Endothelial Cell Growth Media under the conditions suggested by iXCells Biotechnologies. Further expansion may decrease the purity.

Figure 1. Human Brain Microvascular Endothelial Cells (HBMVEC). The cells were recovered, and seeded at 10,000 cells/cm²  following iXCells’ protocol. Phase contrast images were taken at the indicated time post recovery.

Figure 2. Immunofluorescence staining of HBMVEC with antibodies against CD31 (Green), VE-Cadherin (Red) and vWF (Green). Nuclei were counterstained by DAPI (Blue).

Product Details

References

[1] Abbott, N. J., Patabendige, A. A., Dolman, D. E., Yusof, S. R., & Begley, D. J. (2010). Structure and function of the blood-brain barrier. Neurobiology of disease, 37(1), 13–25.

[2] Yang, C., Hawkins, K. E., Doré, S., & Candelario-Jalil, E. (2019). Neuroinflammatory mechanisms of blood-brain barrier damage in ischemic stroke. American journal of physiology. Cell physiology, 316(2), C135–C153.

[3] Godinho-Pereira, J., Garcia, A. R., Figueira, I., Malhó, R., & Brito, M. A. (2021). Behind Brain Metastases Formation: Cellular and Molecular Alterations and Blood-Brain Barrier Disruption. International journal of molecular sciences, 22(13), 7057.

Datasheet & Culture Protocol

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• Alghamri, M. S., Banerjee, K., Mujeeb, A. A., Mauser, A., Taher, A., Thalla, R., McClellan, B. L., Varela, M. L., Stamatovic, S. M., Martinez-Revollar, G., Andjelkovic, A. V., Gregory, J. V., Kadiyala, P., Calinescu, A., Jiménez, J. A., Apfelbaum, A. A., Lawlor, E. R., Carney, S., Comba, A., … Castro, M. G. (2022). Systemic delivery of an adjuvant CXCR4–CXCL12 signaling inhibitor encapsulated in synthetic protein nanoparticles for glioma immunotherapy. ACS Nano, 16(6), 8729–8750. https://doi.org/10.1021/acsnano.1c07492 — Learn More

• Ranjan, N., Pandey, V., Panigrahi, M. K., Klumpp, L., Naumann, U., & Babu, P. P. (2021). The Tumor Suppressor MTUS1/ATIP1 Modulates Tumor Promotion in Glioma: Association with Epigenetics and DNA Repair. Cancers, 13(6), 1245. — Learn More

• Shima, A., Nagata, S., & Takeuchi, S. (2020). Three-dimensional co-culture of BLOOD-BRAIN BARRIER-COMPOSING cells in a culture insert with A COLLAGEN Vitrigel membrane. In Vitro Cellular & Developmental Biology – Animal, 56(7), 500-504. doi:10.1007/s11626-020-00486-x — Learn More
• Cui, X., Ma, C., Vasudevaraja, V., Serrano, J., Tong, J., Peng, Y., . . . Chen, W. (2020). Dissecting the immunosuppressive tumor microenvironments in glioblastoma-on-a-chip for optimized pd-1 immunotherapy. ELife, 9. doi:10.7554/elife.52253 — Learn More
• Heo, J., Kim, J., Woo, S., Kim, K., Choi, K., Lee, H., & Kim, K. (2019). Stromal Cell-Derived factor 1 PROTECTS Brain vascular endothelial cells FROM radiation-induced brain damage. Cells, 8(10), 1230. doi:10.3390/cells8101230 — Learn More

• Hunter, L. W., Jayachandran, M., & Miller, V. M. (2019). Sex differences in the expression of cell adhesion molecules on microvesicles derived from cultured human brain microvascular endothelial cells treated with inflammatory and thrombotic stimuli. Biology of Sex Differences, 10(1). doi:10.1186/s13293-019-0241-y — Learn More

• Ozek, C., Krolewski, R. C., Buchanan, S. M., & Rubin, L. L. (2018). Growth differentiation FACTOR 11 treatment leads to neuronal and Vascular improvements in the hippocampus of aged mice. Scientific Reports, 8(1). doi:10.1038/s41598-018-35716-6 — Learn More

• The cells have been growing for an additional 2 passages upon recovery without antibiotics before release.
• The endothelial cell-specific markers, including CD31, VE-Cadherin, and vWF, maintain >90% purity within 2-3 passages upon recovery.