Description
Product Description
Human Aortic Endothelial Cells (HAOEC) line the vessel wall of aorta, the largest artery in the human body. Because HAOECs are constantly exposed to high hemodynamic forces, they produce endothelium-derived substances regulating vasoconstriction and vessel growth [1]. HAOEC also modulate the expression of cellular adhesion molecules to control and fine-tune inflammatory responses and fibrinolysis [2]. These physiological properties allow HAOEC cultures to be widely used in the study of mechanisms for endothelium dysfunction, pathogenesis of vascular diseases and atherosclerosis, and the development of novel disease treatments.
iXCells Biotechnologies provides high quality HAOEC, which are isolated from human aorta and cryopreserved at P2, with >0.5 million cells in each vial. These HAOEC express vWF/Factor VIII and CD31 (Figure 1). They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can further expand in Endothelial Cell Medium (Cat# MD-0010) under the condition suggested by iXCells Biotechnologies.
Figure 1. iXCells HAOECs are positive for vVF and CD31.
| Protocols
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| Thawing of Frozen Cells
1. Upon receipt of the frozen cells, it is recommended to thaw the cells and initiate the culture immediately in order to retain the highest cell viability. 2. To thaw the cells, put the vial in 37°C water bath with gentle agitation for ~1 minute. Keep the cap out of water to minimize the risk of contamination. 3. Pipette the cells into a 15ml conical tube with 5ml fresh culture medium. 4. Centrifuge at 1000rpm (~220g) for 5 minutes under room temperature. 5. Remove the supernatant and resuspend the cells in fresh culture medium. 6. Culture the cell in T75 flask.
Safety Precaution: it is highly recommended that protective gloves and clothing should be used when handling frozen vials.
Standard Culture Procedure 1. HAOEC can be cultured in Endothelial Cell Medium. 2. When cells reach ~80-90% confluence, remove the medium, and wash once with sterile PBS (5ml/T75 flask). 3. Add ~2.5ml of 0.25% Trypsin-EDTA to the flask and incubate for ~3 minutes at 37°C. Neutralize the enzyme by adding 2-3 volumes of cell culture medium. 4. Centrifuge 1000rpm (~220g) for 5min and resuspend the cells in desired volume of medium. 5. 5. Seed new culture vessels at 5 × 103 cells/cm2. |
Product Details
| Tissue | Human aorta |
| Package Size | 1.0×106 cells/vial |
| Passage Number | P2 |
| Shipped | Cryopreserved |
| Storage | Liquid nitrogen |
| Growth Properties | Adherent |
| Media | Endothelial Cell Medium Kit (Cat# MD-0010) |
References
- Ando J, and Kamiya A. Flow-dependent regulation of gene expression in vascular endothelial cells. Heart J. 1996; 37:19-32.
- Kim M, and Lee YJ. The effect of oxidized low-density lipoprotein (ox-LDL) on radiation-induced endothelial-to-mesenchymal transition. Int J Radiat Biol. 2013; 89(5):356-363.
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