human Myoblasts (iPSC-derived, normal)

Description

Product Description

iPSC-derived myoblasts are valuable tools for biochemical analysis, disease modelling.

iXCells Biotechnologies is proud to provide commercial human iPSC-derived myoblasts for differentiation into functional myotubes. iXCells™ hiPSC-derived myoblasts express typical markers, e.g. MyoD and Desmin (Figure 1) and rapidly differentiate into functional myotubes expressing markers including MHC, Dystrophin and MyoG (Figure 2), with the purity higher than 85%. Functional validation of iPSC-derived myotubes can be observed by their spontaneous twitching in the well.

iXCells™ myoblasts are available in a kit format containing both cryopreserved vials (1 or 3 million cells/vial) along with the necessary media components to expand, differentiate and maintain the myotubes in culture for at least 4-6 weeks.  Longevity of the myotubes in culture can be extended with a bi-weekly 24-hr pulse of Myoblast Expansion Medium followed by replacement with Myotube Differentiation Medium.

iXCells also provide customized differentiation service with your own iPS cell lines.  Please contact us at orders@amogene.com for more details.

Figure 1.  MyoD and Desmin staining of iPSC-derived myoblasts upon recovery in Myoblast Expansion Medium.

Figure 2.  Formation of myotubes were observed after 4 days of differentiation of iPSC-derived myoblasts in Myotube Differentiation Medium. MHC staining of myotubes were shown by immunofluorescence staining (red).

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