Human Liver Non-Parenchymal Cells (HLNPC)

Product Description

The liver is generally comprised of hepatocytes, which make up approximately two thirds of its total cell population (60%-70%), and non parenchymal cells (NPC, 30%-40%). The population of NPC includes liver sinusoidal endothelial cells (LSEC), Kupffer cells, lymphocytes, biliary cells, and hepatic stellate cells (HSCs) [1] . Heterotypic interactions between parenchymal and NPC are important in liver development, physiology, and pathophysiology. Liver NPC participate in liver growth with respect to both their own proliferation, and effects on hepatocyte proliferation. Liver NPC also play crucial roles in the pathogenesis of alcoholic liver disease (ALD) or Nonalcoholic steatohepatitis (NASH) by producing inflammatory mediators such as cytokines, oxidative stress, microRNA, and lipid-originated metabolites or by directly interacting with parenchymal cells [2] [3] .

iXCells Biotechnologies offers Human Liver NPC which were isolated from liver organs of adult donors, and cryopreserved immediately after isolation. Delivered liver NPC with ≥1.0 million cells in each vial have a varying amount of the different non-parenchymal cells with native cell ratio. They are most suitable for liver co-culture applications. They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi.

Figure 1. Phase contrast microscopic images of Human Liver NPC. Liver NPC were plated on Collagen Type 1 coated dish at seeding density of 200K/cm2 and cultured in the presence of Liver NPC Growth Medium (MD-0121). Images were taken at the indicated timepoints post recovery.

References

[1] Malik R, Selden C, Hodgson H. The role of non-parenchymal cells in liver growth. Semin Cell. Dev Biol. 2002 13(6),S.425–431.

[2] Seo W, Jeong WI. Hepatic non-parenchymal cells: Master regulators of alcoholic liver disease? World J Gastroenterol. 2016 Jan 28;22(4):1348-1356.

[3] Loft A, Alfaro AJ, Schmidt SF, Pedersen FB, Terkelsen MK, Puglia M, Chow KK, Feuchtinger A, Troullinaki M, Maida A, Wolff G, Sakurai M, Berutti R, Ekim Üstünel B, Nawroth P, Ravnskjaer K, Diaz MB, Blagoev B, Herzig S. Liver-fibrosis activated transcriptional netw