Human Colonic Epithelial Cells (HCoEpC)

Product Description

The colorectum is a major organ in both malignant and nonmalignant diseases. Cells that line the colonic mucosal surface form a major mechanical barrier that separates the host’s internal milieu from the external environment. In addition to the well-established role of epithelial cells in ion transport, these cells appear to function as an integral component of the mucosal immune system. Human colonic epithelial cells (HCoEpC) can process and present antigens to T cells in vitro, and can be stimulated to express HLA class II and intercellular adhesion molecules in vivo [1]. They also respond to a broad array of cytokines with altered gene expression and growth characteristics [2]. In addition, normal colonic epithelial cells were found to express the α3, α5, α6, β1 and β4 integrin [3].

iXCells Biotechnologies provides high quality HCoEpC, which are isolated from human colonic tissue and cryopreserved at P1, with >0.5 million cells in each vial. HCoEpC express cytokeratin-18 and -19. They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can further expand for 10 population doublings in Epithelial Cell Growth Medium (Cat# MD-0041) under the condition suggested by iXCells Biotechnologies.

Product Details

References

[1] Mayer, L., Eisenhardt, D., Salomon, P., Bauer, W., Plous, R. and Piccinini, L. (1991) Expression of class II molecules on intestinal epithelial cells in humans. Differences between normal and inflammatory bowel disease. Gastroenterology. 100:3-12.
[2] Eckmann, L., Jung H.-C., Schuerer-Maly, C.-C., Panja, A., Morzycka-Wroblewska, E., and Kagnoff, M. F. (1993) Differential cytokine expression by human intestinal epithelial cell lines: regulated expression of interleukin-8. Gastroenterology. 105:1689-1697.
[3] Stallmach, A., v Lampe, B., Matthes, H., Bornhoft, B., and Riecken, E. O. (1992) Diminished expression of integrin adhesion molecules on human colonic epithelial cells during the benign to malign tumor. Gut 33:342-346.

• Cell can be sub-cultured for at least 2 additional passages. 
• Strict QC test 
• High cell purity 
• Cells have been growing for additional 2 passages upon recovery without antibiotics before release;​
• The colonic epithelial cell specific marker MUC2, and the tight junction marker ZO-1, maintain >90% purity within 2-3 passages upon recovery.