Human Hepatic Sinusoidal Endothelial Cells (HHSEC)

Product Description

The human liver contains two distinct types of endothelial cells: vascular endothelial cells and sinusoidal endothelial cells. Human Hepatic Sinusoidal Endothelial Cells (HHSECs) are a specialized subset of microvascular endothelial cells that exhibit a distinct phenotype compared to other endothelial cell types. HHSECs function as antigen-presenting cells, facilitating antigen presentation to CD4+ T cells, and play a crucial role in regulating the immune response within the liver ^[1]. Additionally, HHSECs contribute actively to liver repair by serving as dynamic regulators that respond promptly and locally to zonal environmental stimuli ^[2].

iXCells Biotechnologies provides high quality HHSECs, which are isolated from human liver and cryopreserved at P2, with ≥ 0.5 million cells in each vial. These HHSECs express CD31/PECAM-1, VE-Cadherin and von Willebrand Factor (vWF). They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi, and can be further expanded for no more than 3 passages under the conditions suggested by iXCells Biotechnologies. Further expansion may decrease the purity.

Figure 1.  Phase contrast images of Human Hepatic Sinusoidal Endothelial Cells (HHSEC, Adult). The cells were recovered and seeded at 10,000 cells/cm2 following iXCells’ protocol. The images were taken at the indicated time post-recovery.

Figure 2. Immunofluorescence staining of HHSECs using antibodies against CD31/PECAM-1 (green), VE-Cadherin (red), and vWF (green). The nuclei were counterstained by DAPI (blue).

Product Details

References

[1] Limmer A, and Knolle PA. Liver sinusoidal endothelial cells: a new type of organ-resident antigen-presenting cell. Arch Immunol Ther Exp (Warsz) 2001; 49 (1): S7-11.

[2] Wach KE etc. Sinusoidal ultrastructure evaluated during the revascularization of regenerating rat liver. Hepatoloty 2001; 33 (2): 363-378.

Datasheet & Culture Protocol

Datasheet & Culture Protocol

• Jamil, M. A., Singer, H., Al-Rifai, R., Nüsgen, N., Rath, M., Strauss, S., . . . El-Maarri, O. (2020). Molecular analysis of fetal and adult primary human liver sinusoidal endothelial cells: A comparison to other endothelial cells. International Journal of Molecular Sciences, 21(20), 7776. doi:10.3390/ijms21207776 — Learn More

• • • The cells have been growing for an additional 2 passages upon recovery without antibiotics before release;​
    • The endothelial cell-specific markers, including CD31, VE-Cadherin, and vWF, maintain >90% purity within 2-3 passages upon recovery.