Human Intestinal Smooth Muscle Cells (HISMC)

Product Description

Smooth muscle contraction is the fundamental event in gastrointestinal motility. Although many biochemical mechanisms in excitation-contraction coupling remain undefined, cytosolic Ca²⁺ is recognized as an essential factor in this process. Inflammation of the human intestine leads to increased levels of smooth muscle-specific actin, promoting thickening of the smooth muscle layers. The increased smooth muscle actin may affect force production and further demonstrates the plasticity of smooth muscle cells in the inflamed intestine ^[1]. Studies also show that human intestinal smooth muscle cells (HISMCs) respond to IL-1β and TNF-α stimulation by releasing IL-6, which may significantly contribute to the overall systemic inflammatory response. ^[2]. The availability of HISMCs facilitates research into the contractile and proliferative tissue responses of human intestinal smooth muscle.

iXCells Biotechnologies provides high-quality HISMCs isolated from human intestine and cryopreserved at P1, with ≥ 0.5 million cells in each vial. These HISMCs express α-smooth muscle actin. They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can be further expanded for no more than 3 passages under the conditions suggested by iXCells Biotechnologies. Further expansion may decrease the purity.

Figure 1.Phase contrast images of human Intestinal Smooth Muscle Cells (HISMCs). The cells were recovered and seeded at 10,000 cells/cm2 following iXCells’ protocol. The images were taken at the indicated time post recovery.

Figure 2.Immunofluorescence staining of HISMCs using the antibody against α-smooth muscle actin (green), with nuclei counterstained by DAPI (blue).

Product Details

References

[1] Blennerhassett, M. G., Bovell, F. M., Lourenssen, S., McHugh, K. M. (1999) Characteristics of inflammation-induced hypertrophy of rat intestinal smooth muscle cell. Dig Dis Sci. 44(7):1265-72.

[2] Nallamshetty S, Ng, E. K., Panesar, N., Longo, W. E., Shapiro, M. J., Kaminski, D. L., Tolman, K. C., Mazuski, J. E. (2003) Human intestinal smooth muscle cells are potent producers of IL-6. Mediators Inflamm. 12(1):3-8.

Datasheet & Culture Protocol

• The cells have been growing for additional 3 passages upon recovery without antibiotics before release;​
• The smooth muscle cell specific marker, α-SMA, maintains>90% purity within 2-3 passages upon recovery.