Human Meningeal Cells (HMC)

Product Description

Meningeal cells surround the brain and actively participate in the development of the central nervous system. They play an important role in stabilizing the extracellular matrix of the pial surface, organizing the radial glial scaffold, and laminating the cerebellar cortex ^[1]. Selective pharmacological destruction of the meningeal cells during a critical ontogenetic period leads to specific malformation of both the cerebellar cortex and dentate gyrus ^[1]. Grafts of meningeal cells, which are derived from meninges overlying the cerebral cortex, in adult rat spinal cord lesions promotes axonal regrowth ^[2]. Additionally, in vitro studies showed that meningeal cells chemotactically orient the migration of immature neurons but not glial cells ^[3].

iXCells Biotechnologies provides high quality Human Meningeal Cells (HMC), which are isolated from human leptomeninges and cryopreserved at P0, with >0.5 million cells in each vial. HMC express fibronectin and are negative for GFAP, α-smooth muscle actin, and Thy-1. These HMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can further expand in ​Meningeal CellGrowth Medium (Cat# MD-0050) under the condition suggested by iXCells Biotechnologies.

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References

[1] Hartmann, D., Sievers, J. Pehlemann, F. W. and Berry, M. (1992) Destruction of meningeal cells over the medial cerebral hemisphere of newborn hamster prevents the formation of the infrapyramidal blade of the dentate gyrus. J. Comparative Neurol. 320:33-61.

[2] Franzen, R., Martin, D., Daloze, A., Moonen, G. and Schoenen, J. (1999) Grafts of meningeal fibroblasts in adult rat spinal cord lesion promote axonal regrowth. Neuroreport 10:1551-1556.

[3] Hartmann, D., Schulze, M. and Sievers, J. (1998) Meningeal cells stimulate and direct the migration of cerebellar external granule cells in vitro. J. Neurocytol. 27:395-409.

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