Human Pulmonary Fibroblasts (HPF)

Product Description

The most abundant cell type in lung interstitial is fibroblasts. They resemble ordinary fibroblasts but have some distinguishing features, such as long branching processes and gap junctions. The main function of Human Pulmonary Fibroblasts (HPF) is to produce type III collagen, elastin, and proteoglycans of the extracellular matrix of the alveolar septa. HPF play an important role in the repair and remodeling processes following injury. The controlled accumulation of fibroblasts to sites of inflammation is crucial to effective tissue repair after injury ^[1]. Either inadequate or excessive accumulation of fibroblasts could result in abnormal tissue function. For example, the excess proliferation of fibroblasts contributes to the adventitial thickening observed during the development of hypoxia-induced pulmonary hypertension ^[2].

iXCells Biotechnologies provides high quality Human Pulmonary Fibroblasts (HPF), which are isolated from human lung tissue and cryopreserved at P1, with >0.5 million cells in each vial. HPF express fibronectin and are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. They can further expand in Fibroblast Growth Medium (Cat# MD-0011) for no more than 3 passages under the condition suggested by iXCells Biotechnologies. Further expansion may decrease the proliferation rate and purity.

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Figure 1. Human Pulmonary Fibroblasts (HPF). (A) Phase contrast image of HPF. (B) Immunofluorescence staining with antibody against Vimentin.

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References

[1] Kuwano K, Hagimoto N, Hara N. (2001) Molecular mechanisms of pulmonary fibrosis and current treatment. Curr Mol Med 1(5):551-73.

[2] Das M, Dempsey EC, Reeves JT, Stenmark KR. (2002) Selective expansion of fibroblast subpopulations from pulmonary artery adventitia in response to hypoxia. Am J Physiol Lung Cell Mol Physiol 282(5):L976-86.

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