10HU-007
Human Adipose Derived Stem Cells (ADSCs, Type 2 Diabetes)
Cryopreserved, 0.5 million cells/vial
产品与技术资料CELL CULTURE / PRODUCT
人肠道微血管内皮细胞
Human Intestinal Microvascular Endothelial Cells (HIMEC)
- 产品代码
- 10HU-065
- 产品类别
- 人源原代细胞
- 现有规格
- Cryopreserved, 0.5 million cells/vial
原产品简介与规格资料
产品代码: 10HU-065
人肠道微血管内皮细胞
价格有浮动，详情咨询 (+86) 15750729215
产品规格
选择产品规格:
Cryopreserved, 0.5 million cells/vial
01 / PRODUCT INFORMATION
产品介绍
Product Description
Endothelial cells lining the microvasculature are known to play a critical “gatekeeper” role in the inflammatory process through their ability to recruit circulating immune cells into tissues and foci of inflammation. Studies show that intestinal microvascular endothelial cells (IMEC) exhibit a strong immune response to LPS challenge and play a critical regulatory role in gut inflammation [1]. Pharmacological inhibition of NOS in activated HIMEC resulted in a significant increase in leukocyte binding [2]. Gene expression profile studies revealed that intestinal endothelial cells express biotinidase, which is involved in biotin recycling [3]. HIMEC cultures have enabled scientists to perform systematic analyses of cytokine profiles with regard to mRNA expression and protein secretion, and to compare these data with cytokine profiles from other endothelial cells.
iXCells Biotechnologies provides high quality HIMEC, which are isolated from human intestinal tissue and cryopreserved at P1, with >0.5 million cells in each vial. HIMEC express vWF/Factor VIII, CD31 (PECAM), and Dil-Ac-LDL by uptake. They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can further expand for 10 population doublings in Endothelial Cell Growth Medium (Cat# MD-0010) under the condition suggested by iXCells Biotechnologies.
Product Details
References [1]. Ogawa, H., Rafiee, P., Heidemann, J., Fisher, P. J., Johnson, N. A., Otterson, M. F., Kalyanaraman, B., Pritchard, K. A. Jr., Binion, D. G. (2003) Mechanisms of endotoxin tolerance in human intestinal microvascular endothelial cells. J Immunol. 170(12):5956-64. [2]. Binion, D. G., Fu, S., Ramanujam, K. S., Chai, Y. C., Dweik, R. A., Drazba, J. A., Wade, J. G., Ziats, N. P., Erzurum, S. C. and Wilson, K. T. (1998) iNOS xpression in human intestinal microvascular endothelial cells inhibits leukocyte adhesion. Am. J. Physiol. 275:G592-603. [3]. Chi, J-T, Chang, H. Y., Haraldsen, G., Jahnsen, F. L., Troyanskaya, O. G., Chang, D. S., Wang, Z., Rockson, S. G., van de Rijn, M., Botstein, D. and Brown, P. O. (2003) Endothelial cell diversity revealed by global expression profiling. PNAS 100(19):10623-10628.
Product Details
| Tissue | Human intestinal tissue |
| Package Size | 0.5 million cells/vial |
| Passage Number | P1 |
| Shipped | Cryopreserved |
| Storage | Liquid nitrogen |
| Growth Properties | Adherent |
| Media | Endothelial Cell Growth Medium (Cat# MD-0010) |
References [1]. Ogawa, H., Rafiee, P., Heidemann, J., Fisher, P. J., Johnson, N. A., Otterson, M. F., Kalyanaraman, B., Pritchard, K. A. Jr., Binion, D. G. (2003) Mechanisms of endotoxin tolerance in human intestinal microvascular endothelial cells. J Immunol. 170(12):5956-64. [2]. Binion, D. G., Fu, S., Ramanujam, K. S., Chai, Y. C., Dweik, R. A., Drazba, J. A., Wade, J. G., Ziats, N. P., Erzurum, S. C. and Wilson, K. T. (1998) iNOS xpression in human intestinal microvascular endothelial cells inhibits leukocyte adhesion. Am. J. Physiol. 275:G592-603. [3]. Chi, J-T, Chang, H. Y., Haraldsen, G., Jahnsen, F. L., Troyanskaya, O. G., Chang, D. S., Wang, Z., Rockson, S. G., van de Rijn, M., Botstein, D. and Brown, P. O. (2003) Endothelial cell diversity revealed by global expression profiling. PNAS 100(19):10623-10628.
02 / PRODUCT INFORMATION
产品说明
Product Description
Endothelial cells lining the microvasculature are known to play a critical “gatekeeper” role in the inflammatory process through their ability to recruit circulating immune cells into tissues and foci of inflammation. Studies show that intestinal microvascular endothelial cells (IMEC) exhibit a strong immune response to LPS challenge and play a critical regulatory role in gut inflammation [1]. Pharmacological inhibition of NOS in activated HIMEC resulted in a significant increase in leukocyte binding [2]. Gene expression profile studies revealed that intestinal endothelial cells express biotinidase, which is involved in biotin recycling [3]. HIMEC cultures have enabled scientists to perform systematic analyses of cytokine profiles with regard to mRNA expression and protein secretion, and to compare these data with cytokine profiles from other endothelial cells.
iXCells Biotechnologies provides high quality HIMEC, which are isolated from human intestinal tissue and cryopreserved at P1, with >0.5 million cells in each vial. HIMEC express vWF/Factor VIII, CD31 (PECAM), and Dil-Ac-LDL by uptake. They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can further expand no more than 3 passages in Endothelial Cell Growth Media under the condition suggested by iXCells Biotechnologies. Further expansion may decrease the cell purity.
查看原资料图片：Human Intestinal Microvascular Endothelial Cells HIMEC ↗
Figure legend: (A) Phase contrast image of Human Intestinal Microvascular Endothelial Cells (HIMEC) at DIV4. (B, C) HIMEC are positive for CD31 (B) and vWF/Factor VIII (C) as shown by immunofluorescence staining.
Product Details
| Tissue | Normal human intestinal tissue |
| Package Size | 0.5 million cells/vial |
| Passage Number | P1 |
| Shipped | Cryopreserved |
| Storage | Liquid nitrogen |
| Growth Properties | Adherent |
| Media | Endothelial Cell Growth Media |
References
[1] Jennifer Y Kasper, Maria Iris Hermanns, Christian Cavelius, Annette Kraegeloh, Thomas Jung, Rolf Danzebrink, Ronald E Unger, Charles James Kirkpatrick. (2016) “The role of the intestinal microvasculature in inflammatory bowel disease: studies with a modified Caco-2 model including endothelial cells resembling the intestinal barrier in vitrohe role of the intestinal microvasculature in inflammatory bowel disease: studies with a modified Caco-2 model including endothelial cells resembling the intestinal barrier in vitro”. Int J Nanomedicine. 11:6353-6364.
[2] David G. Binion, Sidong Fu, Kalathur S. Ramanujam, Yuh Cherng Chai, Raed A. Dweik, Judith A. Drazba, Justin G. Wade, Nicholas P. Ziats, Serpil C. Erzurum, and Keith T. Wilson. (1998) “iNOS expression in human intestinal microvascular endothelial cells inhibits leukocyte adhesion”. Physilogy. 275: G592-G603.
[3] E M Nilsena, Johansen, F L Jahnsen, K E A Lundin, T Scholz, P Brandtzaeg, G Haraldsen. (1998) Cytokine profiles of cultured microvascular endothelial cells from the human intestine. 42: 635-642
03 / PRODUCT INFORMATION
资料下载
04 / PRODUCT INFORMATION
文献与引用
- Eddy, J., Pham, F., Chee, R., Park, E., Dapprich, N., DeRuiter, S. L., & Shen, A. (2023). Intestinal endothelial cells increase HIV infection and latency in resting and activated CD4 + T cells, particularly affecting CCR6 + cd4 + T cells. Retrovirology, 20(1). https://doi.org/10.1186/s12977-023-00621-y -- Learn More
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