10HU-007
Human Adipose Derived Stem Cells (ADSCs, Type 2 Diabetes)
Cryopreserved, 0.5 million cells/vial
产品与技术资料CELL CULTURE / PRODUCT
Human Brain Microvascular Endothelial Cells (HBMVEC)
- 产品代码
- 10HU-051
- 产品类别
- 人源原代细胞
- 现有规格
- Cryopreserved, 0.5 million cells/vial
原产品简介与规格资料
产品代码: 10HU-051
人源脑微血管内皮细胞
价格有浮动，详情咨询 (+86) 15750729215
产品规格
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Cryopreserved, 0.5 million cells/vial
01 / PRODUCT INFORMATION
产品介绍
Product Description
The human brain microvascular endothelial cells (HBMVEC) are the major element of the blood-brain barrier and comprise the primary limitation to passage of substances, both soluble and cellular, from the blood into the brain. HBMVEC utilizes unique features that distinguish themselves from those of peripheral endothelial cells. Most prominent among these are the following: 1) intercellular ‘tight junctions’ that display high transendothelial electrical resistance and retard paracellular flux [1], 2) absence of fenestrae and a reduced level of fluid-phase endocytosis [2], and 3) asymmetrically-localized enzymes and carrier-mediated transport systems that engender a truly ‘polarized’ phenotype [3]. Like peripheral endothelial cells, however, HBMVEC express, or can be induced to express, cell adhesion molecules on their surface that regulate the extravasation of leukocytes into the brain. HBMVEC has been widely used for studying the molecular and cellular basis of blood-brain barrier.
iXCells Biotechnologies provides high quality HBMVEC, which are isolated from human brain and cryopreserved at P1, with >0.5 million cells in each vial. These HBMVEC express vWF/Factor VIII, CD31 (PECAM), and Dil-Ac-LDL by uptake. They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can further expand in Endothelial Cell Growth Medium (Cat# MD-0010) under the condition suggested by iXCells Biotechnologies.
查看原资料图片：Human Brain Microvascular Endothelial Cells (HBMVEC) ↗
Figure 1. Immunostaining of Human Brain Microvascular Endothelial Cells (HBMVEC) using the antibodies against vWF (green) and CD31 (red).
Product Details
References [1] Crone, C. and Oleson, S. P. (1992) Electrical resistance of brain microvessel endothelium. Brain Res. 241: 49-55. [2] Reese, T. S. and Karnovsky, M. J. (1967) Fine structural localization of blood-brain barrier to exogenous peroxidase. J. Cell Biol. 34:9-14. [3] Vorbrodt, A. W. (1988) Ultrastructural cytochemistry of blood-brain barrier endothelia. Prog. Histochem. Cytochem. 18(3):1-96.
Product Details
| Tissue | Human brain tissue |
| Package Size | 0.5 million cells/vial |
| Passage Number | P1 |
| Shipped | Cryopreserved |
| Storage | Liquid nitrogen |
| Growth Properties | Adherent |
| Media | Endothelial Cell Growth Medium (Cat# MD-0010) |
References [1] Crone, C. and Oleson, S. P. (1992) Electrical resistance of brain microvessel endothelium. Brain Res. 241: 49-55. [2] Reese, T. S. and Karnovsky, M. J. (1967) Fine structural localization of blood-brain barrier to exogenous peroxidase. J. Cell Biol. 34:9-14. [3] Vorbrodt, A. W. (1988) Ultrastructural cytochemistry of blood-brain barrier endothelia. Prog. Histochem. Cytochem. 18(3):1-96.
02 / PRODUCT INFORMATION
产品说明
Product Description
Human Brain Microvascular Endothelial Cells (HBMVEC) are the major element of the blood-brain barrier that shields the brain against toxins and immune cells via paracellular, transcellular, transporter, and extracellular matrix proteins [1]. HBMVEC are morphologically different from the peripheral endothelium. Brain endothelial cells lack fenestrations, have minimal pinocytic activity, are connected by tight junctions and have a large number of mitochondria associated with high metabolic activity [2]. Like peripheral endothelial cells, however, HBMVEC express, or can be induced to express, cell adhesion molecules on their surface that regulate the extravasation of leukocytes into the brain. HBMVEC have been widely used for studying the molecular and cellular function of blood-brain barrier [3].
iXCells Biotechnologies provides high quality HBMVEC, which are isolated from human brain and cryopreserved at P2, with ≥ 0.5 million cells in each vial. These HBMVEC express von Willebrand Factor (vWF), CD31 (PECAM), and Dil-Ac-LDL by uptake. They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can be further expanded for no more than 3 passages in Endothelial Cell Growth Media under the conditions suggested by iXCells Biotechnologies. Further expansion may decrease the purity.
Figure 1. Human Brain Microvascular Endothelial Cells (HBMVEC). The cells were recovered, and seeded at 10,000 cells/cm2 following iXCells’ protocol. Phase contrast images were taken at the indicated time post recovery.
Figure 2. Immunofluorescence staining of HBMVEC with antibodies against CD31 (Green), VE-Cadherin (Red) and vWF (Green). Nuclei were counterstained by DAPI (Blue).
Product Details
| Organism | Homo Sapiens, Human |
| Cell Type | Endothelial Cell |
| Tissue | Human Brain |
| Disease | Normal |
| Package Size | 0.5 x 106 cells/vial |
| Passage Number | P2 |
| Growth Properties | Adherent |
| Product Format/Shipped | Cryopreserved |
| Storage | Liquid Nitrogen |
| Associated Media | Endothelial Cell Growth Media |
References
[1] Abbott, N. J., Patabendige, A. A., Dolman, D. E., Yusof, S. R., & Begley, D. J. (2010). Structure and function of the blood-brain barrier. Neurobiology of disease, 37(1), 13–25.
[2] Yang, C., Hawkins, K. E., Doré, S., & Candelario-Jalil, E. (2019). Neuroinflammatory mechanisms of blood-brain barrier damage in ischemic stroke. American journal of physiology. Cell physiology, 316(2), C135–C153.
[3] Godinho-Pereira, J., Garcia, A. R., Figueira, I., Malhó, R., & Brito, M. A. (2021). Behind Brain Metastases Formation: Cellular and Molecular Alterations and Blood-Brain Barrier Disruption. International journal of molecular sciences, 22(13), 7057.
03 / PRODUCT INFORMATION
资料下载
04 / PRODUCT INFORMATION
文献与引用
Bioz 实时文献组件 · 可在组件内筛选和滚动浏览；无法载入时可在 Bioz 打开。
Alghamri, M. S., Banerjee, K., Mujeeb, A. A., Mauser, A., Taher, A., Thalla, R., McClellan, B. L., Varela, M. L., Stamatovic, S. M., Martinez-Revollar, G., Andjelkovic, A. V., Gregory, J. V., Kadiyala, P., Calinescu, A., Jiménez, J. A., Apfelbaum, A. A., Lawlor, E. R., Carney, S., Comba, A., … Castro, M. G. (2022). Systemic delivery of an adjuvant CXCR4–CXCL12 signaling inhibitor encapsulated in synthetic protein nanoparticles for glioma immunotherapy. ACS Nano, 16(6), 8729–8750. https://doi.org/10.1021/acsnano.1c07492 -- Learn More
Ranjan, N., Pandey, V., Panigrahi, M. K., Klumpp, L., Naumann, U., & Babu, P. P. (2021). The Tumor Suppressor MTUS1/ATIP1 Modulates Tumor Promotion in Glioma: Association with Epigenetics and DNA Repair. Cancers, 13(6), 1245. -- Learn More
- Shima, A., Nagata, S., & Takeuchi, S. (2020). Three-dimensional co-culture of BLOOD-BRAIN BARRIER-COMPOSING cells in a culture insert with A COLLAGEN Vitrigel membrane. In Vitro Cellular & Developmental Biology - Animal, 56(7), 500-504. doi:10.1007/s11626-020-00486-x -- Learn More
- Cui, X., Ma, C., Vasudevaraja, V., Serrano, J., Tong, J., Peng, Y., . . . Chen, W. (2020). Dissecting the immunosuppressive tumor microenvironments in glioblastoma-on-a-chip for optimized pd-1 immunotherapy. ELife, 9. doi:10.7554/elife.52253 -- Learn More
- Heo, J., Kim, J., Woo, S., Kim, K., Choi, K., Lee, H., & Kim, K. (2019). Stromal Cell-Derived factor 1 PROTECTS Brain vascular endothelial cells FROM radiation-induced brain damage. Cells, 8(10), 1230. doi:10.3390/cells8101230 -- Learn More
- Hunter, L. W., Jayachandran, M., & Miller, V. M. (2019). Sex differences in the expression of cell adhesion molecules on microvesicles derived from cultured human brain microvascular endothelial cells treated with inflammatory and thrombotic stimuli. Biology of Sex Differences, 10(1). doi:10.1186/s13293-019-0241-y -- Learn More
Ozek, C., Krolewski, R. C., Buchanan, S. M., & Rubin, L. L. (2018). Growth differentiation FACTOR 11 treatment leads to neuronal and Vascular improvements in the hippocampus of aged mice. Scientific Reports, 8(1). doi:10.1038/s41598-018-35716-6 -- Learn More
05 / PRODUCT INFORMATION
Product Highlights
- The cells have been growing for an additional 2 passages upon recovery without antibiotics before release.
- The endothelial cell-specific markers, including CD31, VE-Cadherin, and vWF, maintain >90% purity within 2-3 passages upon recovery.
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