Description
Product Description
The CD8+ T cells, also known as Cytotoxic T cells (TC cells), are a type of T cells that kill cancer cells, cells that are infected (particularly with viruses), or cells that are damaged in other ways. Most cytotoxic T cells express T-cell receptors (TCRs) that can recognize a specific antigen, and a glycoprotein called CD8 which binds to the constant portion of the class I MHC molecule. When exposed to infected/dysfunctional somatic cells, CD8+ T cells release the cytotoxins perforin, granzymes, and granulysin, which triggers the caspase cascade and eventually leads to apoptosis. CD8+ T cells have been implicated in the pathogenesis of hepatitis B virus infection [1], arthritis [2] etc.
iXCells Biotechnologies offers CD8+ T cells isolated from normal human peripheral blood mononuclear cells (PBMCs) using negative immunomagnetic selection. > 90% of the cells are CD8+ as showed by flow cytometric analysis (Figure 1).
Figure 1. Flow cytometric analysis showed that >90% of the cells are CD8+.
Product Details
| Tissue | Normal human peripheral blood |
| Package Size | 5.0×106 cells/vial, 1.0×107 cells/vial |
| Purity | >90% |
| Passage Number | P0 |
| Shipped | Cryopreserved |
| Storage | Liquid nitrogen |
| Growth Properties | Suspension |
| Media | Blood Cell Culture Medium (Cat# MD-0007) |
| Protocols |
| Thawing of Frozen Cells
1. Upon receipt of the frozen CD8+ T cells, it is recommended to thaw the cells and initiate the culture immediately in order to retain the highest cell viability. 2. To thaw the cells, put the vial in 37°C water bath with gentle agitation for 1-2 minutes. Keep the cap out of water to minimize the risk of contamination. 3. Pipette the cells into a 15 mL conical tube with 5 mL fresh Blood Cell Culture Medium (Cat# MD-0007). 4. Centrifuge at 400-450 g for 5 minutes under room temperature. 5. Remove the supernatant and cell is ready for downstream applications. Safety Precaution: it is highly recommended that protective gloves and clothing should be used when handling frozen vials. |
References
[1] Iannacone, Matteo; Sitia, Giovanni; Guidotti, Luca G (2006). “Pathogenetic and antiviral immune responses against hepatitis B virus”. Future Virology 1 (2): 189–96. [2] Subramanian S and Ramalingam K (2005). “Electron microscopic evidence on the participation Cytotoxic T Lymphocytes and Macrophages in Mtb adjuvant induced connective tissue inflammation and arthritogenesis in Rattus norvegicus”. Asian Journal of Microbiology, Biotechnology and Environmental Sciences 7 (2): 227–233.
Reviews
There are no reviews yet.