Human Peripheral Blood CD19+ B Cells (Untouched)

Product Description

B cells, also known as B lymphocytes, are a subtype of lymphocyte in white blood cells ^[1]. They play a critical role in the humoral immunity component of the adaptive immune system by secreting antibodies^[1]. They also function in immune system as antigen presentation cells and by secretion of cytokines^[1].

CD19 (Cluster of Differentiation 19) is an important surface marker for B cells from earliest recognizable B-lineage cells during development to B-cell blasts but is lost on maturation to plasma cells. CD19 primarily acts as a B cell co-receptor in conjunction with CD21 ^[2] and CD81. Upon activation, the cytoplasmic tail of CD19 becomes phosphorylated, which leads to binding by Src-family kinases and recruitment of PI-3 kinase.

iXCells Biotechnologies offers CD19+ B Cells isolated from normal human peripheral blood mononuclear cells (PBMCs) using negative immunomagnetic selection. > 90% of the cells are CD19+ as showed by flow cytometric analysis.

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References

[1]  Murphy, Kenneth. 2012. Janeway’s Immunobiology 8^th Edition. New York, NY: Garland Science.

[2]  Bradbury LE, Kansas GS, Levy S, Evans RL, and Tedder TF. “The CD19/CD21 signal transducing complex of human B lymphocytes includes the target of antiproliferative antibody-1 and Leu-13 molecules”. Journal of Immunology 1992; 149 (9):2841-2850.

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Manuals & Protocols

• Hansen, M. H., Cédile, O., Blum, M. K., Hansen, S. V., Ebbesen, L. H., Bentzen, H. H., . . . Nyvold, C. G. (2020). Molecular characterization of sorted malignant b cells from patients clinically identified with mantle cell lymphoma. Experimental Hematology, 84. doi:10.1016/j.exphem.2020.03.001 — Learn More

• Van Gent, M., Reich, A., Velu, S. E., & Gack, M. U. (2021). Nonsense-mediated decay controls the reactivation of the oncogenic herpesviruses EBV And kshv. PLOS Biology, 19(2). doi:10.1371/journal.pbio.3001097 – Learn More