Human Pancreatic Microvascular Endothelial Cells (HPaMEC)

Product Overview

The pancreas functions as both an endocrine gland and a digestive organ, secreting hormones and producing pancreatic juice to aid in digestion.^[1,2] The pancreatic islets, which serve as the endocrine component of the pancreas, produce essential hormones such as glucagon, insulin, and amylin ^[1,2]. Human Pancreatic Microvascular Endothelial Cells (HPaMECs) play a critical role in supporting the islets by delivering oxygen and nutrients to pancreatic tissue, influencing beta cell function and proliferation, and regulating insulin gene expression during islet development. Additionally, HPaMECs produce various growth factors, optimize blood glucose sensing, and help regulate blood glucose levels. HPaMECs can serve as an in vitro model for studying islet biology, pancreatic cancer, transplantation, and regenerative medicine.

iXCells Biotechnologies provides high quality HPaMECs, which are isolated from human pancreas and cryopreserved at P2, with ≥ 0.5 million cells in each vial. These HPaMECs express von Willebrand Factor (vWF), VE-Cadherin and CD31/PECAM-1. They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi, and can be further expanded for no more than 3 passages under the conditions suggested by iXCells Biotechnologies. Further expansion may decrease the purity.

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Figure 1.Phase contrast images of Human Pancreatic Microvascular Endothelial Cells (HPaMECs). The cells were recovered and seeded at 10,000 cells/cm2 following iXCells’ protocol. The images were taken at the indicated time post recovery.

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Figure 2.Immunofluorescence staining of HPaMECs using antibodies against VE-Cadherin (green), vWF (green) and CD31/PECAM-1 (green) with nuclei counterstained by DAPI (blue).

Product Details

References

[1] Zanone M, Favaro E, Camussi G (2008). “From endothelial to beta cells: insights into pancreatic islet microendothelium.” Curr Diabetes Rev. 4(1): 1-9.
[2] Konstantinova I and Lammert E. (2004). “Microvascular development: learning from pancreatic islets.” Bioessays, 26(10):1069-1075.

Datasheet & Culture Protocol

• The cells have been growing for an additional 2 passages upon recovery without antibiotics before release;
• Upon recovery, the endothelial cell specific markers, including CD31, VE-Cadherin, and vWF, maintain >90% purity within 2-3 passages.