{"id":3409,"date":"2026-04-10T15:56:25","date_gmt":"2026-04-10T07:56:25","guid":{"rendered":"https:\/\/biomobio.com\/?page_id=3409"},"modified":"2026-04-10T16:09:58","modified_gmt":"2026-04-10T08:09:58","slug":"human-gecko-v2-crispr-screen","status":"publish","type":"page","link":"https:\/\/biomobio.com\/?page_id=3409","title":{"rendered":"CRISPR Screen"},"content":{"rendered":"\t\t
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\n\t\t\t\r\n\r\n\r\n \r\n \r\n Human GeCKO v2 CRISPR sgRNA Screening | \u5168\u57fa\u56e0\u7ec4\u6572\u9664\u7b5b\u9009\u670d\u52a1<\/title>\r\n <meta name=\"description\" content=\"Human GeCKO v2 \u5168\u57fa\u56e0\u7ec4 CRISPR\/Cas9 \u6572\u9664\u7b5b\u9009\u670d\u52a1\uff0c\u8986\u76d6 19,050 \u4e2a\u4eba\u7c7b\u57fa\u56e0\u4e0e 123,411 \u6761 gRNA\uff0c\u652f\u6301 pooled CRISPR screening\u3001NGS \u6570\u636e\u5206\u6790\u3001\u5019\u9009\u57fa\u56e0\u6392\u5e8f\u4e0e\u901a\u8def\u89e3\u6790\u3002\">\r\n <style>\r\n #gecko-screen-page{--bg:#ffffff;--bg-soft:#f8fbf2;--paper:#ffffff;--paper-2:#fbfdf7;--ink:#243220;--soft:#66745d;--line:rgba(36,50,32,.10);--brand:#7bc043;--brand-deep:#5aa338;--accent:#ffc433;--accent-deep:#ff9800;--olive:#bfc3ab;--shadow:0 20px 48px rgba(62,86,34,.10);--r-xl:28px;--r-lg:20px;--r-md:14px;--max:1200px;--space:84px;margin:0;font-family:\"PingFang SC\",\"Microsoft YaHei\",\"Noto Sans SC\",sans-serif;color:var(--ink);background:linear-gradient(180deg,#ffffff 0%,#fbfdf8 100%)}\r\n #gecko-screen-page,#gecko-screen-page *{box-sizing:border-box}\r\n #gecko-screen-page a{text-decoration:none;color:inherit}\r\n #gecko-screen-page img,#gecko-screen-page svg{display:block;max-width:100%}\r\n #gecko-screen-page h1,#gecko-screen-page h2,#gecko-screen-page h3,#gecko-screen-page h4,#gecko-screen-page p{margin:0}\r\n #gecko-screen-page .container{width:min(var(--max),calc(100% - 40px));margin:0 auto}\r\n #gecko-screen-page .section{padding:var(--space) 0}\r\n #gecko-screen-page .eyebrow{display:inline-flex;align-items:center;gap:10px;padding:8px 14px;border-radius:999px;background:rgba(123,192,67,.08);border:1px solid rgba(123,192,67,.16);color:var(--brand-deep);font-size:12px;font-weight:700;letter-spacing:.14em;text-transform:uppercase}\r\n #gecko-screen-page .eyebrow:before{content:\"\";width:8px;height:8px;border-radius:50%;background:linear-gradient(135deg,var(--brand),var(--accent))}\r\n #gecko-screen-page .btn-row{display:flex;gap:14px;flex-wrap:wrap}\r\n #gecko-screen-page .btn{display:inline-flex;align-items:center;justify-content:center;min-height:48px;padding:0 22px;border-radius:999px;font-weight:700;border:1px solid transparent;transition:.2s ease}\r\n #gecko-screen-page .btn:hover{transform:translateY(-1px)}\r\n #gecko-screen-page .btn.primary{background:linear-gradient(135deg,var(--brand),var(--accent));color:#283213;box-shadow:0 16px 32px rgba(174,194,53,.22)}\r\n #gecko-screen-page .btn.secondary{background:#fff;border-color:rgba(123,192,67,.24);color:var(--brand-deep)}\r\n #gecko-screen-page .btn.ghost{background:#fff;border-color:var(--line);color:var(--brand-deep)}\r\n #gecko-screen-page .site-header{position:sticky;top:0;z-index:40;background:rgba(255,255,255,.94);backdrop-filter:blur(16px);border-bottom:1px solid 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rgba(123,192,67,.22);color:var(--brand-deep);font-weight:800;font-size:18px;box-shadow:var(--shadow);margin-bottom:16px}\r\n #gecko-screen-page .step-body{padding:18px;border-radius:18px;background:#fff;border:1px solid var(--line);box-shadow:var(--shadow);min-height:190px}\r\n #gecko-screen-page .step-body h3{font-size:18px;line-height:1.35;margin-bottom:10px}\r\n #gecko-screen-page .step-body p,#gecko-screen-page .item p{color:var(--soft);font-size:14px;line-height:1.8}\r\n #gecko-screen-page .item{padding:16px 18px;border-radius:16px}\r\n #gecko-screen-page .item strong{display:block;margin-bottom:6px;font-size:15px}\r\n #gecko-screen-page .chart{background:linear-gradient(180deg,#fffef8,#f9fbee)}\r\n #gecko-screen-page .faq{display:grid;gap:12px}\r\n #gecko-screen-page .faq-item{border-radius:18px;overflow:hidden}\r\n #gecko-screen-page .faq-q{width:100%;border:0;background:transparent;color:var(--ink);padding:20px 22px;text-align:left;display:flex;align-items:center;justify-content:space-between;gap:16px;font:inherit;font-size:16px;font-weight:700;cursor:pointer}\r\n #gecko-screen-page .faq-q span:last-child{font-size:22px;color:var(--brand-deep);transition:transform .25s ease;line-height:1}\r\n #gecko-screen-page .faq-item.open .faq-q span:last-child{transform:rotate(45deg)}\r\n #gecko-screen-page .faq-a{display:grid;grid-template-rows:0fr;transition:grid-template-rows .28s ease}\r\n #gecko-screen-page .faq-item.open .faq-a{grid-template-rows:1fr}\r\n #gecko-screen-page .faq-a-inner{overflow:hidden}\r\n #gecko-screen-page .faq-a p{padding:0 22px 20px;color:var(--soft);font-size:15px;line-height:1.85}\r\n #gecko-screen-page .cta{padding-top:16px}\r\n #gecko-screen-page .cta-shell{padding:34px;border-radius:30px;border:1px solid rgba(123,192,67,.14);background:linear-gradient(180deg,#fffef9,#f5faeb);box-shadow:var(--shadow);display:flex;align-items:center;justify-content:space-between;gap:20px;flex-wrap:wrap}\r\n #gecko-screen-page .cta-copy h2{font-size:clamp(28px,3.2vw,44px);line-height:1.1;margin-bottom:10px;max-width:14ch}\r\n #gecko-screen-page .cta-copy p{color:var(--soft);font-size:16px;line-height:1.8;max-width:620px}\r\n #gecko-screen-page .site-footer{padding:26px 0 42px;border-top:1px solid var(--line);color:var(--soft);font-size:13px}\r\n #gecko-screen-page .foot{display:flex;justify-content:space-between;gap:18px;flex-wrap:wrap}\r\n #gecko-screen-page .fade{opacity:0;transform:translateY(14px);animation:gecko-rise .7s ease forwards}\r\n #gecko-screen-page .delay-1{animation-delay:.08s}\r\n @keyframes gecko-rise{to{opacity:1;transform:translateY(0)}}\r\n @media (max-width:1120px){#gecko-screen-page .hero-grid,#gecko-screen-page .intro-shell,#gecko-screen-page .analysis-shell,#gecko-screen-page .grid-4,#gecko-screen-page .timeline{grid-template-columns:1fr}#gecko-screen-page .hero-grid{grid-template-areas:\"title\" \"copy\" \"media\"}#gecko-screen-page .grid-6{grid-template-columns:repeat(2,minmax(0,1fr))}#gecko-screen-page .hero-points{grid-template-columns:1fr}#gecko-screen-page .timeline:before{display:none}#gecko-screen-page .step-body{min-height:auto}}\r\n @media (max-width:760px){#gecko-screen-page{--space:68px}#gecko-screen-page .container{width:min(var(--max),calc(100% - 28px))}#gecko-screen-page .nav{padding:12px 0}#gecko-screen-page .menu{gap:12px}#gecko-screen-page .section-head{flex-direction:column;align-items:start}#gecko-screen-page .grid-2,#gecko-screen-page .grid-3,#gecko-screen-page .grid-6,#gecko-screen-page .hero-mini{grid-template-columns:1fr}#gecko-screen-page .hero{padding-top:52px}#gecko-screen-page .hero-title h1{white-space:normal;font-size:clamp(30px,10vw,46px)}#gecko-screen-page .cta-shell{padding:24px}}\r\n <\/style>\r\n<\/head>\r\n<body>\r\n <div id=\"gecko-screen-page\">\r\n <header class=\"site-header\">\r\n <div class=\"container nav\">\r\n <a class=\"brand\" href=\"#top\">\r\n <strong>CRISPR SCREENING SERVICE<\/strong>\r\n <span>Human GeCKO v2 \u5168\u57fa\u56e0\u7ec4\u6572\u9664\u7b5b\u9009\u670d\u52a1<\/span>\r\n <\/a>\r\n <nav class=\"menu\">\r\n <a href=\"#intro\">\u6280\u672f\u7b80\u4ecb<\/a>\r\n <a href=\"#features\">\u6587\u5e93\u7279\u70b9<\/a>\r\n <a href=\"#services\">\u670d\u52a1\u5185\u5bb9<\/a>\r\n <a href=\"#applications\">\u5e94\u7528\u573a\u666f<\/a>\r\n <a href=\"#workflow\">\u6807\u51c6\u6d41\u7a0b<\/a>\r\n <a href=\"#analysis\">\u6570\u636e\u5206\u6790<\/a>\r\n <a href=\"#faq\">FAQ<\/a>\r\n <a href=\"#contact\" class=\"btn ghost\">\u83b7\u53d6\u65b9\u6848<\/a>\r\n <\/nav>\r\n <\/div>\r\n <\/header>\r\n\r\n <main id=\"top\">\r\n <section class=\"hero\">\r\n <div class=\"container hero-grid\">\r\n <div class=\"hero-title fade\">\r\n <span class=\"eyebrow\">Genome-Scale Pooled CRISPR Knockout Screening<\/span>\r\n <h2>Human GeCKO v2 \u5168\u57fa\u56e0\u7ec4 CRISPR\/Cas9 \u6572\u9664\u7b5b\u9009\u670d\u52a1<\/h2>\r\n <\/div>\r\n <div class=\"hero-copy fade\">\r\n <p class=\"lead\">\r\n \u57fa\u4e8e Feng Zhang \u56e2\u961f Human GeCKO v2 pooled CRISPR knockout screening \u6587\u5e93\uff0c\r\n \u9762\u5411\u4eba\u7c7b\u5168\u57fa\u56e0\u7ec4\u8303\u56f4\u5f00\u5c55\u9ad8\u901a\u91cf loss-of-function \u529f\u80fd\u7b5b\u9009\uff0c\r\n \u9002\u7528\u4e8e\u836f\u7269\u54cd\u5e94\u3001\u4f9d\u8d56\u57fa\u56e0\u3001\u8010\u836f\u673a\u5236\u3001\u529f\u80fd\u57fa\u56e0\u548c\u5019\u9009\u9776\u70b9\u6316\u6398\u3002\r\n \u670d\u52a1\u4e0d\u4ec5\u8986\u76d6\u7b5b\u9009\u5b9e\u9a8c\u5b9e\u65bd\uff0c\u540c\u65f6\u63d0\u4f9b NGS \u6570\u636e\u5206\u6790\u3001\u5019\u9009\u57fa\u56e0\u6392\u5e8f\u548c\u901a\u8def\u89e3\u6790\u3002\r\n <\/p>\r\n <div class=\"hero-tags\">\r\n <span class=\"hero-tag\">\u5168\u57fa\u56e0\u7ec4\u7b5b\u9009<\/span>\r\n <span class=\"hero-tag\">\u652f\u6301 A \/ B \u534a\u5e93\u4e0e A+B \u5168\u5e93<\/span>\r\n <span class=\"hero-tag\">\u63d0\u4f9b NGS \u89e3\u5377\u79ef\u4e0e hit \u6392\u5e8f<\/span>\r\n <\/div>\r\n <div class=\"btn-row\" style=\"margin-top:28px\">\r\n <a class=\"btn primary\" href=\"#contact\">\u8054\u7cfb\u6211\u4eec \/ \u83b7\u53d6\u65b9\u6848<\/a>\r\n <a class=\"btn secondary\" href=\"#workflow\">\u67e5\u770b\u6807\u51c6\u6d41\u7a0b<\/a>\r\n <\/div>\r\n <div class=\"hero-points\">\r\n <div class=\"hero-stat\"><strong>19,050<\/strong><span>\u8986\u76d6 19,050 \u4e2a\u4eba\u7c7b\u57fa\u56e0\uff0c\u9002\u7528\u4e8e genome-scale \u529f\u80fd\u7b5b\u9009\u3002<\/span><\/div>\r\n <div class=\"hero-stat\"><strong>123,411<\/strong><span>\u603b\u8ba1 123,411 \u6761 gRNA\uff0c\u6ee1\u8db3 pooled CRISPR screening \u8986\u76d6\u9700\u6c42\u3002<\/span><\/div>\r\n <div class=\"hero-stat\"><strong>\u4e00\u7ad9\u5f0f<\/strong><span>\u4ece\u7b5b\u9009\u8bbe\u8ba1\u5230 NGS \u6570\u636e\u5206\u6790\u3001\u57fa\u56e0\u6392\u5e8f\u4e0e\u901a\u8def\u89e3\u6790\u5b8c\u6574\u4ea4\u4ed8\u3002<\/span><\/div>\r\n <\/div>\r\n <\/div>\r\n\r\n <aside class=\"hero-media fade delay-1\">\r\n <img decoding=\"async\" src=\"https:\/\/biomobio.com\/wp-content\/uploads\/2025\/04\/%E6%9C%AA%E6%A0%87%E9%A2%98-1-03.jpg\" alt=\"Human GeCKO v2 CRISPR \u7b5b\u9009\u76f8\u5173\u793a\u610f\u56fe\">\r\n <strong>GeCKO \u5168\u57fa\u56e0\u7ec4 CRISPR \u6572\u9664\u7b5b\u9009\u6280\u672f\u793a\u610f\u56fe<\/strong>\r\n <div class=\"hero-mini\">\r\n <div class=\"mini\">\r\n <strong>\u7b5b\u9009\u5b9e\u65bd<\/strong>\r\n <p>\u8986\u76d6\u6587\u5e93\u611f\u67d3\u3001\u7b5b\u9009\u6761\u4ef6\u65bd\u52a0\u3001\u6837\u672c\u6784\u5efa\u4e0e\u540e\u7eed\u6d4b\u5e8f\u8ba1\u6570\u6d41\u7a0b\u3002<\/p>\r\n <\/div>\r\n <div class=\"mini\">\r\n <strong>\u7ed3\u679c\u8f93\u51fa<\/strong>\r\n <p>\u4e0d\u4ec5\u63d0\u4f9b\u5b9e\u9a8c\uff0c\u8fd8\u8f93\u51fa NGS \u5206\u6790\u3001\u5019\u9009\u57fa\u56e0\u6392\u5e8f\u548c\u901a\u8def\u5c42\u9762\u7684\u89e3\u91ca\u3002<\/p>\r\n <\/div>\r\n <\/div>\r\n <\/aside>\r\n <\/div>\r\n <\/section>\r\n <section id=\"intro\" class=\"section\">\r\n <div class=\"container\">\r\n <div class=\"section-head\">\r\n <div><span class=\"eyebrow\">Technical Overview<\/span><h2>\u6280\u672f\u7b80\u4ecb<\/h2><\/div>\r\n <p>\u5c06 Human GeCKO v2 pooled sgRNA \u6587\u5e93\u5bfc\u5165\u7ec6\u80de\u7fa4\u4f53\uff0c\u5728\u836f\u7269\u5904\u7406\u3001\u589e\u6b96\u7ade\u4e89\u3001\u5b58\u6d3b\u538b\u529b\u6216\u8868\u578b\u5206\u9009\u7b49\u6761\u4ef6\u4e0b\u6bd4\u8f83\u7b5b\u9009\u524d\u540e sgRNA \u4e30\u5ea6\u53d8\u5316\uff0c\u4ece\u800c\u5728\u5168\u57fa\u56e0\u7ec4\u8303\u56f4\u8bc6\u522b\u4e0e\u76ee\u6807\u8868\u578b\u76f8\u5173\u7684\u5173\u952e\u57fa\u56e0\u4e0e\u901a\u8def\u3002<\/p>\r\n <\/div>\r\n <div class=\"intro-shell\">\r\n <div class=\"card\">\r\n <h3>Human GeCKO v2 pooled CRISPR knockout screening<\/h3>\r\n <p>Human GeCKO v2 \u7531 Feng Zhang \u56e2\u961f\u5f00\u53d1\uff0c\u9762\u5411\u4eba\u7c7b\u5168\u57fa\u56e0\u7ec4\u529f\u80fd\u7b5b\u9009\u3002\u670d\u52a1\u56f4\u7ed5 pooled knockout screen \u8bbe\u8ba1\u3001\u611f\u67d3\u4e0e\u7b5b\u9009\u5b9e\u65bd\u3001NGS \u6570\u636e\u5206\u6790\u3001\u5019\u9009\u57fa\u56e0\u6392\u5e8f\u548c\u901a\u8def\u89e3\u6790\u5c55\u5f00\uff0c\u9002\u7528\u4e8e\u9700\u8981\u7cfb\u7edf\u8bc6\u522b\u529f\u80fd\u57fa\u56e0\u4e0e\u8c03\u63a7\u901a\u8def\u7684\u9879\u76ee\u3002<\/p>\r\n <p style=\"margin-top:14px\">\u5bf9\u4f9d\u8d56\u57fa\u56e0\u3001\u836f\u7269\u54cd\u5e94\u3001\u8010\u836f\u673a\u5236\u4e0e\u5019\u9009\u9776\u70b9\u6316\u6398\u7b49\u9879\u76ee\uff0c\u8be5\u8def\u5f84\u80fd\u591f\u63d0\u4f9b\u66f4\u9ad8\u901a\u91cf\u3001\u66f4\u7cfb\u7edf\u7684\u529f\u80fd\u53d1\u73b0\u80fd\u529b\u3002<\/p>\r\n <\/div>\r\n <div class=\"diagram\">\r\n <span class=\"badge\">\u7b5b\u9009\u4e3b\u7ebf<\/span>\r\n <div class=\"steps\">\r\n <div class=\"step\"><div class=\"num\">1<\/div><span>Human GeCKO v2 \u6587\u5e93\u611f\u67d3\u7ec6\u80de\u7fa4\u4f53\uff0c\u5efa\u7acb pooled knockout \u521d\u59cb\u6c60\u3002<\/span><\/div>\r\n <div class=\"step\"><div class=\"num\">2<\/div><span>\u65bd\u52a0\u836f\u7269\u3001\u5b58\u6d3b\u3001\u589e\u6b96\u6216\u5206\u9009\u538b\u529b\uff0c\u5f62\u6210\u76ee\u6807\u8868\u578b\u5dee\u5f02\u3002<\/span><\/div>\r\n <div class=\"step\"><div class=\"num\">3<\/div><span>\u6269\u589e sgRNA \u533a\u57df\u5e76\u8fdb\u884c NGS\uff0c\u6bd4\u8f83\u7b5b\u9009\u524d\u540e\u4e30\u5ea6\u53d8\u5316\u3002<\/span><\/div>\r\n <div class=\"step\"><div class=\"num\">4<\/div><span>\u5728\u57fa\u56e0\u5c42\u9762\u8bc6\u522b\u5bcc\u96c6 \/ \u8017\u7aed\u4fe1\u53f7\uff0c\u8f93\u51fa hits \u6392\u5e8f\u4e0e\u901a\u8def\u89e3\u6790\u7ed3\u679c\u3002<\/span><\/div>\r\n <\/div>\r\n <\/div>\r\n <\/div>\r\n <\/div>\r\n <\/section>\r\n\r\n <section id=\"features\" class=\"section\">\r\n <div class=\"container\">\r\n <div class=\"section-head\">\r\n <div><span class=\"eyebrow\">Library Features<\/span><h2>Human GeCKO v2 \u6587\u5e93\u7279\u70b9<\/h2><\/div>\r\n <p>\u6587\u5e93\u8bbe\u8ba1\u56f4\u7ed5\u4eba\u7c7b\u5168\u57fa\u56e0\u7ec4\u529f\u80fd\u7b5b\u9009\u5c55\u5f00\uff0c\u517c\u987e\u57fa\u56e0\u8986\u76d6\u5e7f\u5ea6\u3001gRNA \u6570\u91cf\u548c\u5b9e\u9a8c\u7b56\u7565\u7075\u6d3b\u6027\uff0c\u9002\u5408\u4ece\u534a\u5e93\u5230\u5168\u5e93\u7684\u4e0d\u540c\u7b5b\u9009\u9700\u6c42\u3002<\/p>\r\n <\/div>\r\n <div class=\"grid-4\">\r\n <div class=\"metric\"><strong>19,050<\/strong><span>\u4eba\u7c7b\u57fa\u56e0<\/span><p>\u9762\u5411 19,050 \u4e2a\u4eba\u7c7b\u57fa\u56e0\u5f00\u5c55 genome-scale loss-of-function \u529f\u80fd\u7b5b\u9009\u3002<\/p><\/div>\r\n <div class=\"metric\"><strong>123,411<\/strong><span>\u6761 gRNA<\/span><p>\u603b\u8ba1 123,411 \u6761 gRNA\uff0c\u4e3a pooled CRISPR screen \u63d0\u4f9b\u9ad8\u89c4\u6a21\u8986\u76d6\u80fd\u529b\u3002<\/p><\/div>\r\n <div class=\"metric\"><strong>A \/ B<\/strong><span>\u53cc\u534a\u5e93\u8bbe\u8ba1<\/span><p>\u652f\u6301 A\u3001B \u4e24\u4e2a\u534a\u5e93\u72ec\u7acb\u4f7f\u7528\uff0c\u4e5f\u53ef\u7ec4\u5408\u4e3a A+B \u5168\u5e93\u65b9\u6848\u3002<\/p><\/div>\r\n <div class=\"metric\"><strong>1,000<\/strong><span>\u5bf9\u7167 \/ \u534a\u5e93<\/span><p>\u6bcf\u4e2a\u534a\u5e93\u5747\u542b 1,000 \u6761 non-targeting controls\uff0c\u4fbf\u4e8e\u7b5b\u9009\u8d28\u63a7\u4e0e\u7edf\u8ba1\u5f52\u4e00\u5316\u3002<\/p><\/div>\r\n <\/div>\r\n <\/div>\r\n <\/section>\r\n\r\n <section id=\"services\" class=\"section\">\r\n <div class=\"container\">\r\n <div class=\"section-head\">\r\n <div><span class=\"eyebrow\">Service Scope<\/span><h2>\u670d\u52a1\u5185\u5bb9<\/h2><\/div>\r\n <p>\u670d\u52a1\u4e0d\u6b62\u4e8e pooled CRISPR knockout \u7b5b\u9009\u672c\u8eab\uff0c\u8fd8\u8986\u76d6 NGS \u89e3\u5377\u79ef\u3001\u5019\u9009\u57fa\u56e0\u4f18\u5148\u7ea7\u6392\u5e8f\u4e0e\u901a\u8def\u5c42\u9762\u7684\u7ed3\u679c\u89e3\u6790\u3002<\/p>\r\n <\/div>\r\n <div class=\"grid-2\">\r\n <article class=\"card\"><span class=\"service-badge\">01 \u6838\u5fc3\u7b5b\u9009<\/span><h3>Human GeCKO v2 \u5168\u57fa\u56e0\u7ec4 KO \u521d\u7b5b<\/h3><p>\u56f4\u7ed5\u4eba\u7c7b\u5168\u57fa\u56e0\u7ec4\u8303\u56f4\u5f00\u5c55 unbiased discovery\uff0c\u9002\u7528\u4e8e\u4f9d\u8d56\u57fa\u56e0\u3001\u8010\u836f\u673a\u5236\u548c\u529f\u80fd\u57fa\u56e0\u6316\u6398\u9879\u76ee\u3002<\/p><\/article>\r\n <article class=\"card\"><span class=\"service-badge\">02 \u7b56\u7565\u8bbe\u8ba1<\/span><h3>A \/ B \u534a\u5e93\u4e0e A+B \u5168\u5e93\u65b9\u6848\u8bbe\u8ba1<\/h3><p>\u6839\u636e\u7ec6\u80de\u91cf\u3001\u7b5b\u9009\u7a97\u53e3\u3001\u9879\u76ee\u9884\u7b97\u548c\u5b9e\u9a8c\u590d\u6742\u5ea6\uff0c\u9009\u62e9\u534a\u5e93\u6216\u5168\u5e93\u7b56\u7565\uff0c\u964d\u4f4e\u76f2\u76ee\u4e0a\u5e93\u5e26\u6765\u7684\u6210\u672c\u4e0e\u98ce\u9669\u3002<\/p><\/article>\r\n <article class=\"card\"><span class=\"service-badge\">03 \u5b9e\u9a8c\u5b9e\u65bd<\/span><h3>\u611f\u67d3\u3001\u7b5b\u9009\u4e0e\u6837\u672c\u6784\u5efa<\/h3><p>\u8986\u76d6\u6587\u5e93\u611f\u67d3\u3001\u7b5b\u9009\u6761\u4ef6\u5b9e\u65bd\u3001\u6837\u672c\u65f6\u95f4\u70b9\u8bbe\u8ba1\u53ca\u540e\u7eed sgRNA \u6269\u589e\u6837\u672c\u6784\u5efa\uff0c\u5f62\u6210\u7a33\u5b9a\u7684 pooled screening \u6d41\u7a0b\u3002<\/p><\/article>\r\n <article class=\"card\"><span class=\"service-badge\">04 \u6570\u636e\u89e3\u6790<\/span><h3>NGS \u6570\u636e\u5206\u6790\u4e0e\u5019\u9009\u57fa\u56e0\u6392\u5e8f<\/h3><p>\u4ece FASTQ \u5230 sgRNA count\u3001gene ranking\u3001pathway enrichment\uff0c\u8f93\u51fa\u53ef\u76f4\u63a5\u7528\u4e8e\u51b3\u7b56\u7684\u7ed3\u679c\u5c42\u4fe1\u606f\u3002<\/p><\/article>\r\n <\/div>\r\n <\/div>\r\n <\/section>\r\n\r\n <section id=\"applications\" class=\"section\">\r\n <div class=\"container\">\r\n <div class=\"section-head\">\r\n <div><span class=\"eyebrow\">Applications<\/span><h2>\u5e94\u7528\u573a\u666f<\/h2><\/div>\r\n <p>\u9002\u7528\u4e8e\u56f4\u7ed5\u836f\u7269\u53cd\u5e94\u3001\u7ec6\u80de\u751f\u5b58\u3001\u529f\u80fd\u4f9d\u8d56\u548c\u8868\u578b\u53d8\u5316\u5f00\u5c55\u7684\u9ad8\u901a\u91cf\u529f\u80fd\u7b5b\u9009\u9879\u76ee\uff0c\u5e2e\u52a9\u66f4\u5feb\u9501\u5b9a\u5177\u6709\u540e\u7eed\u9a8c\u8bc1\u4ef7\u503c\u7684\u5019\u9009\u57fa\u56e0\u4e0e\u901a\u8def\u3002<\/p>\r\n <\/div>\r\n <div class=\"grid-6\">\r\n <article class=\"card app-card\"><div><div class=\"app-icon\">01<\/div><h3>\u836f\u7269\u8010\u836f\u673a\u5236\u7b5b\u9009<\/h3><p>\u8bc6\u522b\u5bfc\u81f4\u8010\u836f\u8868\u578b\u51fa\u73b0\u6216\u7ef4\u6301\u7684\u5173\u952e\u57fa\u56e0\u4e0e\u901a\u8def\uff0c\u4e3a\u8010\u836f\u673a\u5236\u89e3\u6790\u63d0\u4f9b\u7ebf\u7d22\u3002<\/p><\/div><\/article>\r\n <article class=\"card app-card\"><div><div class=\"app-icon\">02<\/div><h3>\u836f\u7269\u654f\u611f\u6027\u589e\u5f3a\u56e0\u5b50<\/h3><p>\u5bfb\u627e\u80fd\u589e\u5f3a\u836f\u7269\u54cd\u5e94\u6216\u534f\u540c\u836f\u6548\u7684\u8c03\u63a7\u56e0\u5b50\uff0c\u652f\u6301\u8054\u5408\u6cbb\u7597\u4e0e\u9776\u70b9\u7ec4\u5408\u63a2\u7d22\u3002<\/p><\/div><\/article>\r\n <article class=\"card app-card\"><div><div class=\"app-icon\">03<\/div><h3>\u4f9d\u8d56\u57fa\u56e0\u53d1\u73b0<\/h3><p>\u56f4\u7ed5\u7ec6\u80de\u5b58\u6d3b\u3001\u589e\u6b96\u4e0e\u7ade\u4e89\u4f18\u52bf\u8bc6\u522b\u4f9d\u8d56\u57fa\u56e0\uff0c\u670d\u52a1\u4e8e\u80bf\u7624\u4e0e\u75be\u75c5\u6a21\u578b\u7814\u7a76\u3002<\/p><\/div><\/article>\r\n <article class=\"card app-card\"><div><div class=\"app-icon\">04<\/div><h3>\u5408\u6210\u81f4\u6b7b\u9776\u70b9\u63a2\u7d22<\/h3><p>\u5728\u7279\u5b9a\u9057\u4f20\u80cc\u666f\u6216\u836f\u7269\u6761\u4ef6\u4e0b\u6316\u6398\u5408\u6210\u81f4\u6b7b\u76f8\u5173\u5019\u9009\u9776\u70b9\uff0c\u652f\u6301\u7cbe\u7ec6\u5316\u7b5b\u9009\u8bbe\u8ba1\u3002<\/p><\/div><\/article>\r\n <article class=\"card app-card\"><div><div class=\"app-icon\">05<\/div><h3>\u514d\u75ab\u76f8\u5173\u529f\u80fd\u57fa\u56e0\u7b5b\u9009<\/h3><p>\u9002\u7528\u4e8e\u514d\u75ab\u6cbb\u7597\u3001\u514d\u75ab\u9003\u9038\u4e0e\u7ec6\u80de\u8868\u9762\u8868\u578b\u8c03\u63a7\u76f8\u5173\u9879\u76ee\u7684\u529f\u80fd\u57fa\u56e0\u53d1\u73b0\u3002<\/p><\/div><\/article>\r\n <article class=\"card app-card\"><div><div class=\"app-icon\">06<\/div><h3>\u7279\u5b9a\u8868\u578b\u8c03\u63a7\u56e0\u5b50\u6316\u6398<\/h3><p>\u56f4\u7ed5\u51cb\u4ea1\u3001\u5206\u5316\u3001\u8fc1\u79fb\u3001\u5206\u9009\u5bcc\u96c6\u7b49\u76ee\u6807\u8868\u578b\uff0c\u5b9a\u4f4d\u9a71\u52a8\u8868\u578b\u53d8\u5316\u7684\u5173\u952e\u56e0\u5b50\u3002<\/p><\/div><\/article>\r\n <\/div>\r\n <\/div>\r\n <\/section>\r\n <section id=\"workflow\" class=\"section\">\r\n <div class=\"container\">\r\n <div class=\"section-head\">\r\n <div><span class=\"eyebrow\">Workflow<\/span><h2>\u6807\u51c6\u6d41\u7a0b<\/h2><\/div>\r\n <p>\u6309\u9879\u76ee\u63a8\u8fdb\u987a\u5e8f\u642d\u5efa 6 \u6b65\u65f6\u95f4\u7ebf\uff0c\u4ece\u7b5b\u9009\u9700\u6c42\u5230\u7ed3\u679c\u62a5\u544a\u5f62\u6210\u6e05\u6670\u95ed\u73af\uff0c\u4fbf\u4e8e\u5ba2\u6237\u5feb\u901f\u5224\u65ad\u9879\u76ee\u6240\u5904\u9636\u6bb5\u4e0e\u6240\u9700\u8f93\u5165\u3002<\/p>\r\n <\/div>\r\n <div class=\"timeline\">\r\n <article class=\"step-card\"><div class=\"step-dot\">1<\/div><div class=\"step-body\"><h3>\u9700\u6c42\u6c9f\u901a<\/h3><p>\u660e\u786e\u7ec6\u80de\u6a21\u578b\u3001\u76ee\u6807\u8868\u578b\u3001\u7b5b\u9009\u538b\u529b\u3001\u91cd\u590d\u7b56\u7565\u548c\u9879\u76ee\u8f93\u51fa\u76ee\u6807\u3002<\/p><\/div><\/article>\r\n <article class=\"step-card\"><div class=\"step-dot\">2<\/div><div class=\"step-body\"><h3>\u6587\u5e93\u7b56\u7565\u9009\u62e9<\/h3><p>\u6839\u636e\u9879\u76ee\u89c4\u6a21\u4e0e\u6761\u4ef6\u786e\u5b9a A \u534a\u5e93\u3001B \u534a\u5e93\u6216 A+B \u5168\u5e93\u65b9\u6848\u3002<\/p><\/div><\/article>\r\n <article class=\"step-card\"><div class=\"step-dot\">3<\/div><div class=\"step-body\"><h3>\u7ec6\u80de\u6a21\u578b\u4e0e\u611f\u67d3\u4f18\u5316<\/h3><p>\u56f4\u7ed5\u611f\u67d3\u6548\u7387\u3001\u8986\u76d6\u5ea6\u4e0e\u7b5b\u9009\u7a97\u53e3\u4f18\u5316\u5b9e\u9a8c\u8f93\u5165\u6761\u4ef6\u3002<\/p><\/div><\/article>\r\n <article class=\"step-card\"><div class=\"step-dot\">4<\/div><div class=\"step-body\"><h3>\u7b5b\u9009\u5b9e\u65bd<\/h3><p>\u6267\u884c\u836f\u7269\u5904\u7406\u3001\u589e\u6b96\u7ade\u4e89\u3001\u5b58\u6d3b\u538b\u529b\u6216\u5206\u9009\u7b49 pooled screening \u6761\u4ef6\u3002<\/p><\/div><\/article>\r\n <article class=\"step-card\"><div class=\"step-dot\">5<\/div><div class=\"step-body\"><h3>sgRNA \u6d4b\u5e8f<\/h3><p>\u6269\u589e sgRNA \u533a\u57df\u5e76\u5f00\u5c55 NGS\uff0c\u83b7\u5f97\u540e\u7eed\u8ba1\u6570\u4e0e\u5dee\u5f02\u5206\u6790\u6240\u9700\u6570\u636e\u3002<\/p><\/div><\/article>\r\n <article class=\"step-card\"><div class=\"step-dot\">6<\/div><div class=\"step-body\"><h3>\u751f\u4fe1\u5206\u6790\u4e0e\u62a5\u544a<\/h3><p>\u5b8c\u6210\u8ba1\u6570\u3001\u5019\u9009\u57fa\u56e0\u6392\u5e8f\u3001\u901a\u8def\u5bcc\u96c6\u4e0e\u7ed3\u679c\u89e3\u91ca\uff0c\u5f62\u6210\u9879\u76ee\u4ea4\u4ed8\u6750\u6599\u3002<\/p><\/div><\/article>\r\n <\/div>\r\n <\/div>\r\n <\/section>\r\n\r\n <section id=\"analysis\" class=\"section\">\r\n <div class=\"container\">\r\n <div class=\"section-head\">\r\n <div><span class=\"eyebrow\">Bioinformatics<\/span><h2>\u6570\u636e\u5206\u6790<\/h2><\/div>\r\n <p>\u6570\u636e\u5206\u6790\u4e0d\u6b62\u505c\u7559\u5728 reads \u8d28\u63a7\u548c sgRNA \u8ba1\u6570\uff0c\u800c\u662f\u8fdb\u4e00\u6b65\u5b8c\u6210 gene-level ranking\u3001pathway enrichment \u4e0e\u5019\u9009\u7ed3\u679c\u4f18\u5148\u7ea7\u6574\u7406\uff0c\u5e2e\u52a9\u66f4\u5feb\u8fdb\u5165\u540e\u7eed\u9a8c\u8bc1\u9636\u6bb5\u3002<\/p>\r\n <\/div>\r\n <div class=\"analysis-shell\">\r\n <div class=\"card\">\r\n <div class=\"list\">\r\n <div class=\"item\"><strong>1. Reads \u8d28\u63a7<\/strong><p>\u5bf9\u6d4b\u5e8f\u539f\u59cb\u6570\u636e\u8fdb\u884c\u8d28\u91cf\u8bc4\u4f30\uff0c\u786e\u8ba4\u8bfb\u957f\u3001\u8d28\u91cf\u5206\u5e03\u548c\u6837\u672c\u5b8c\u6574\u6027\u3002<\/p><\/div>\r\n <div class=\"item\"><strong>2. sgRNA \u6bd4\u5bf9\u4e0e\u8ba1\u6570<\/strong><p>\u5c06 reads \u6620\u5c04\u81f3 Human GeCKO v2 \u6587\u5e93\u5e8f\u5217\uff0c\u751f\u6210 sgRNA count matrix\u3002<\/p><\/div>\r\n <div class=\"item\"><strong>3. \u5f52\u4e00\u5316\u4e0e\u91cd\u590d\u4e00\u81f4\u6027\u8bc4\u4f30<\/strong><p>\u6bd4\u8f83\u7ec4\u95f4\u5206\u5e03\u4e0e\u91cd\u590d\u76f8\u5173\u6027\uff0c\u5224\u65ad\u7b5b\u9009\u7a97\u53e3\u4e0e\u6570\u636e\u7a33\u5b9a\u6027\u3002<\/p><\/div>\r\n <div class=\"item\"><strong>4. Gene-level ranking<\/strong><p>\u7ed3\u5408\u7ecf\u5178 CRISPR screen \u5206\u6790\u601d\u8def\uff0c\u5bf9\u5bcc\u96c6 \/ \u8017\u7aed\u57fa\u56e0\u8fdb\u884c\u7edf\u8ba1\u6392\u5e8f\u3002<\/p><\/div>\r\n <div class=\"item\"><strong>5. \u901a\u8def\u89e3\u6790\u4e0e hits \u4f18\u5148\u7ea7\u6574\u7406<\/strong><p>\u8f93\u51fa pathway enrichment \u548c\u5019\u9009\u57fa\u56e0\u4f18\u5148\u5217\u8868\uff0c\u63d0\u5347\u7ed3\u679c\u89e3\u91ca\u4e0e\u540e\u7eed\u9a8c\u8bc1\u6548\u7387\u3002<\/p><\/div>\r\n <\/div>\r\n <\/div>\r\n <div class=\"card\">\r\n <span class=\"badge\">\u53ef\u4ea4\u4ed8\u56fe\u8868<\/span>\r\n <div class=\"list\">\r\n <div class=\"item chart\"><strong>Sequencing QC Summary<\/strong><p>\u6d4b\u5e8f\u8d28\u91cf\u7edf\u8ba1\u3001reads \u5206\u5e03\u4e0e\u6837\u672c\u5c42\u8d28\u63a7\u6458\u8981\u3002<\/p><\/div>\r\n <div class=\"item chart\"><strong>sgRNA Count Distribution<\/strong><p>\u5404\u6837\u672c sgRNA \u8ba1\u6570\u5206\u5e03\u3001\u8986\u76d6\u5ea6\u4e0e\u5e93\u590d\u6742\u5ea6\u6982\u89c8\u3002<\/p><\/div>\r\n <div class=\"item chart\"><strong>Sample Correlation Heatmap<\/strong><p>\u91cd\u590d\u4e00\u81f4\u6027\u4e0e\u7ec4\u95f4\u5dee\u5f02\u7684\u76f8\u5173\u6027\u70ed\u56fe\u3002<\/p><\/div>\r\n <div class=\"item chart\"><strong>Gene Ranking Plot<\/strong><p>\u5019\u9009\u57fa\u56e0\u5bcc\u96c6 \/ \u8017\u7aed\u6392\u5e8f\u7ed3\u679c\u4e0e\u663e\u8457\u6027\u5206\u5e03\u3002<\/p><\/div>\r\n <div class=\"item chart\"><strong>Pathway Enrichment Chart<\/strong><p>\u663e\u8457\u901a\u8def\u5bcc\u96c6\u6761\u5f62\u56fe\u3001\u6c14\u6ce1\u56fe\u6216\u7f51\u7edc\u5173\u7cfb\u56fe\u3002<\/p><\/div>\r\n <div class=\"item chart\"><strong>Hit Prioritization Table<\/strong><p>\u5019\u9009\u57fa\u56e0\u4f18\u5148\u7ea7\u6392\u5e8f\u8868\uff0c\u4fbf\u4e8e\u540e\u7eed\u9a8c\u8bc1\u4e0e\u9879\u76ee\u51b3\u7b56\u3002<\/p><\/div>\r\n <\/div>\r\n <\/div>\r\n <\/div>\r\n <\/div>\r\n <\/section>\r\n\r\n <section id=\"deliverables\" class=\"section\">\r\n <div class=\"container\">\r\n <div class=\"section-head\">\r\n <div><span class=\"eyebrow\">Deliverables<\/span><h2>\u4ea4\u4ed8\u5185\u5bb9<\/h2><\/div>\r\n <p>\u4ea4\u4ed8\u56f4\u7ed5\u5b9e\u9a8c\u8fc7\u7a0b\u53ef\u8ffd\u8e2a\u3001\u6570\u636e\u7ed3\u679c\u53ef\u590d\u7528\u3001\u5019\u9009\u57fa\u56e0\u53ef\u51b3\u7b56\u5c55\u5f00\uff0c\u4fbf\u4e8e\u5ba2\u6237\u76f4\u63a5\u8854\u63a5\u5185\u90e8\u590d\u7b5b\u3001\u9a8c\u8bc1\u6216\u540e\u7eed\u673a\u5236\u7814\u7a76\u3002<\/p>\r\n <\/div>\r\n <div class=\"grid-3\">\r\n <div class=\"item\"><strong>\u9879\u76ee\u65b9\u6848\u8bf4\u660e<\/strong><p>\u5305\u542b\u6587\u5e93\u7b56\u7565\u3001\u7b5b\u9009\u903b\u8f91\u3001\u6837\u672c\u8bbe\u8ba1\u548c\u5206\u6790\u6846\u67b6\u6458\u8981\u3002<\/p><\/div>\r\n <div class=\"item\"><strong>\u5b9e\u9a8c\u6d41\u7a0b\u4e0e QC \u6458\u8981<\/strong><p>\u63d0\u4f9b\u9879\u76ee\u6267\u884c\u8fc7\u7a0b\u4e2d\u7684\u5173\u952e\u8282\u70b9\u548c\u57fa\u7840\u8d28\u91cf\u63a7\u5236\u4fe1\u606f\u3002<\/p><\/div>\r\n <div class=\"item\"><strong>FASTQ \u539f\u59cb\u6570\u636e<\/strong><p>\u4ea4\u4ed8\u6d4b\u5e8f\u539f\u59cb\u6587\u4ef6\uff0c\u4fbf\u4e8e\u5185\u90e8\u5b58\u6863\u4e0e\u540e\u7eed\u590d\u6838\u3002<\/p><\/div>\r\n <div class=\"item\"><strong>sgRNA Count Matrix<\/strong><p>\u63d0\u4f9b\u5404\u6837\u672c sgRNA \u8ba1\u6570\u77e9\u9635\uff0c\u7528\u4e8e\u4e0b\u6e38\u7edf\u8ba1\u5206\u6790\u548c\u6570\u636e\u590d\u7528\u3002<\/p><\/div>\r\n <div class=\"item\"><strong>Gene Ranking \u4e0e\u6bd4\u8f83\u7ed3\u679c<\/strong><p>\u8f93\u51fa\u5019\u9009\u57fa\u56e0\u6392\u5e8f\u3001\u5bcc\u96c6 \/ \u8017\u7aed\u8d8b\u52bf\u53ca\u7ec4\u95f4\u6bd4\u8f83\u7ed3\u679c\u3002<\/p><\/div>\r\n <div class=\"item\"><strong>\u901a\u8def\u89e3\u6790\u4e0e\u5b8c\u6574\u62a5\u544a<\/strong><p>\u5305\u542b pathway enrichment \u56fe\u8868\u3001\u5019\u9009\u57fa\u56e0\u5217\u8868\u53ca\u540e\u7eed\u9a8c\u8bc1\u5efa\u8bae\u3002<\/p><\/div>\r\n <\/div>\r\n <\/div>\r\n <\/section>\r\n\r\n <section id=\"faq\" class=\"section\">\r\n <div class=\"container\">\r\n <div class=\"section-head\">\r\n <div><span class=\"eyebrow\">FAQ<\/span><h2>\u5e38\u89c1\u95ee\u9898<\/h2><\/div>\r\n <p>\u9488\u5bf9 Human GeCKO\u3001pooled CRISPR screening\u3001sgRNA screen \u8bbe\u8ba1\u4e0e\u4ea4\u4ed8\u4e2d\u6700\u5e38\u89c1\u7684\u95ee\u9898\u96c6\u4e2d\u8bf4\u660e\u3002<\/p>\r\n <\/div>\r\n <div class=\"faq\">\r\n <div class=\"faq-item open\"><button class=\"faq-q\" type=\"button\"><span>Human GeCKO v2 \u7684 A \/ B \u534a\u5e93\u5e94\u8be5\u600e\u4e48\u9009\uff1f<\/span><span>+<\/span><\/button><div class=\"faq-a\"><div class=\"faq-a-inner\"><p>\u5982\u679c\u9879\u76ee\u53d7\u9650\u4e8e\u7ec6\u80de\u6570\u91cf\u3001\u8986\u76d6\u5ea6\u6216\u9884\u7b97\uff0c\u53ef\u4f18\u5148\u9009\u62e9 A \u6216 B \u5355\u534a\u5e93\uff1b\u5982\u679c\u5e0c\u671b\u83b7\u5f97\u66f4\u5b8c\u6574\u7684\u5168\u57fa\u56e0\u7ec4\u8986\u76d6\u4e0e\u66f4\u9ad8\u7b5b\u9009\u5197\u4f59\u5ea6\uff0c\u53ef\u9009\u62e9 A+B \u5168\u5e93\u7b56\u7565\u3002<\/p><\/div><\/div><\/div>\r\n <div class=\"faq-item\"><button class=\"faq-q\" type=\"button\"><span>\u5355\u534a\u5e93\u548c A+B \u5168\u5e93\u7684\u5dee\u522b\u662f\u4ec0\u4e48\uff1f<\/span><span>+<\/span><\/button><div class=\"faq-a\"><div class=\"faq-a-inner\"><p>\u5355\u534a\u5e93\u66f4\u9002\u5408\u5feb\u901f\u521d\u7b5b\u548c\u8d44\u6e90\u53d7\u9650\u9879\u76ee\uff1bA+B \u5168\u5e93\u5219\u5728 gRNA \u5197\u4f59\u5ea6\u548c\u7b5b\u9009\u8986\u76d6\u4e0a\u66f4\u5145\u5206\uff0c\u9002\u5408\u5bf9\u7ed3\u679c\u7a33\u5065\u6027\u8981\u6c42\u66f4\u9ad8\u7684\u9879\u76ee\u3002<\/p><\/div><\/div><\/div>\r\n <div class=\"faq-item\"><button class=\"faq-q\" type=\"button\"><span>\u505a pooled CRISPR knockout screen \u662f\u5426\u5fc5\u987b\u4f7f\u7528 Cas9 \u7a33\u8f6c\u7ec6\u80de\uff1f<\/span><span>+<\/span><\/button><div class=\"faq-a\"><div class=\"faq-a-inner\"><p>\u591a\u6570 pooled CRISPR\/Cas9 knockout screening \u9879\u76ee\u9700\u8981\u5177\u5907\u7a33\u5b9a Cas9 \u8868\u8fbe\u7684\u7ec6\u80de\u57fa\u7840\uff0c\u4ee5\u786e\u4fdd sgRNA \u5bfc\u5165\u540e\u80fd\u591f\u5b9e\u73b0\u6301\u7eed\u6709\u6548\u7684\u57fa\u56e0\u6572\u9664\u3002<\/p><\/div><\/div><\/div>\r\n <div class=\"faq-item\"><button class=\"faq-q\" type=\"button\"><span>\u8fd9\u7c7b sgRNA screen \u9002\u5408\u54ea\u4e9b\u8868\u578b\uff1f<\/span><span>+<\/span><\/button><div class=\"faq-a\"><div class=\"faq-a-inner\"><p>\u9002\u7528\u4e8e\u836f\u7269\u654f\u611f\u6027 \/ \u8010\u836f\u3001\u589e\u6b96\u7ade\u4e89\u3001\u5b58\u6d3b\u538b\u529b\u3001\u7ec6\u80de\u5206\u9009\u5bcc\u96c6\u3001\u7279\u5b9a\u529f\u80fd\u72b6\u6001\u53d8\u5316\u7b49\u53ef\u8f6c\u5316\u4e3a\u7ec4\u95f4\u5dee\u5f02\u6bd4\u8f83\u7684\u8868\u578b\u573a\u666f\u3002<\/p><\/div><\/div><\/div>\r\n <div class=\"faq-item\"><button class=\"faq-q\" type=\"button\"><span>\u662f\u5426\u63d0\u4f9b\u4ece\u6d4b\u5e8f\u5230\u62a5\u544a\u7684\u4e00\u7ad9\u5f0f\u5206\u6790\uff1f<\/span><span>+<\/span><\/button><div class=\"faq-a\"><div class=\"faq-a-inner\"><p>\u63d0\u4f9b\u3002\u670d\u52a1\u4e0d\u4ec5\u5305\u542b NGS \u6570\u636e\u5904\u7406\u548c sgRNA \u8ba1\u6570\uff0c\u8fd8\u5305\u62ec\u5019\u9009\u57fa\u56e0\u6392\u5e8f\u3001\u901a\u8def\u89e3\u6790\u3001\u56fe\u8868\u6574\u7406\u4e0e\u7ed3\u679c\u62a5\u544a\u8f93\u51fa\u3002<\/p><\/div><\/div><\/div>\r\n <div class=\"faq-item\"><button class=\"faq-q\" type=\"button\"><span>\u521d\u7b5b hits \u4e4b\u540e\u80fd\u5426\u7ee7\u7eed\u505a\u590d\u7b5b\u6216\u9a8c\u8bc1\uff1f<\/span><span>+<\/span><\/button><div class=\"faq-a\"><div class=\"faq-a-inner\"><p>\u53ef\u4ee5\u3002\u4ea4\u4ed8\u7ed3\u679c\u4f1a\u63d0\u4f9b\u5019\u9009\u57fa\u56e0\u4f18\u5148\u7ea7\u6392\u5e8f\u4e0e\u89e3\u6790\u7ebf\u7d22\uff0c\u4fbf\u4e8e\u540e\u7eed\u8bbe\u8ba1\u590d\u7b5b\u3001\u5355\u57fa\u56e0\u9a8c\u8bc1\u6216\u9776\u70b9\u529f\u80fd\u7814\u7a76\u3002<\/p><\/div><\/div><\/div>\r\n <\/div>\r\n <\/div>\r\n <\/section>\r\n\r\n <section id=\"contact\" class=\"section cta\">\r\n <div class=\"container\">\r\n <div class=\"cta-shell\">\r\n <div class=\"cta-copy\">\r\n <span class=\"eyebrow\">Contact<\/span>\r\n <h2>\u8ba9\u5168\u57fa\u56e0\u7ec4 CRISPR \u7b5b\u9009\u66f4\u5feb\u8fdb\u5165\u53ef\u51b3\u7b56\u9636\u6bb5<\/h2>\r\n <p>\u56f4\u7ed5 Human GeCKO v2 pooled CRISPR knockout screening\uff0c\u83b7\u53d6\u9002\u5408\u5f53\u524d\u7ec6\u80de\u6a21\u578b\u3001\u7b5b\u9009\u7a97\u53e3\u4e0e\u9879\u76ee\u76ee\u6807\u7684\u5b9e\u65bd\u65b9\u6848\u3002<\/p>\r\n <\/div>\r\n <div class=\"btn-row\">\r\n <a class=\"btn primary\" 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