Description
Product Description
Dermal Fibroblasts (HDF) are the most prevalent cell in human dermis, and one of the most important architects of cutaneous would healing [1]. The fibroblast is a malleable cell, capable of altering its function and physiology or even transforming into a new cell type, based on its location within the body. The dermal fibroblast also has the unique title of being the first human somatic cell to be induced into a pluripotent stem cell line [2,3].Dermal Fibroblasts (HDF) are the most prevalent cell in human dermis, and one of the most important architects of cutaneous would healing [1]. The fibroblast is a malleable cell, capable of altering its function and physiology or even transforming into a new cell type, based on its location within the body. The dermal fibroblast also has the unique title of being the first human somatic cell to be induced into a pluripotent stem cell line [2,3].
iXCells Biotechnologies provides high quality Human Dermal Fibroblasts (HDF) from either neonatal foreskin (Cat# 10HU-013) or adult skin (Cat# 10HU-014). These cells are derived from the dermis of normal human neonatal foreskin or adult skin and cryopreserved at the end of primary culture. HDF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. They can further expand in Fibroblast Growth Medium (Cat# MD-0011) under the condition suggested by iXCells Biotechnologies. A Certificate of Analysis is provided for each cell lot purchased.
Applications:
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- iPSC generation
- ECM protein analysis
- Wound healing
- Collagen metabolism
- Skin therapy/models
Figure 1. (A) Human Neonatal Dermal Fibroblasts (10HU-013). (B) Human Adult Dermal Fibroblasts (10HU-014).
Product Details
| Tissue | Human Dermal Fibroblasts, Normal (Neonatal foreskin, adult skin) |
| Package Size | 0.5×106 cells/vial, 1.0×106 cells/vial |
| Shipped | Cryopreserved |
| Storage | Liquid nitrogen |
| Growth Properties | Adherent |
| Media | Fibroblast Growth Medium (Cat# MD-0011) |
| Protocols |
| Thawing of Frozen Cells
1. Upon receipt of the frozen cells, it is recommended to thaw the cells and initiate the culture immediately in order to retain the highest cell viability. 2. To thaw the cells, put the vial in 37°C water bath with gentle agitation for ~1 minute. Keep the cap out of water to minimize the risk of contamination. 3. Pipette the cells into a 15ml conical tube with 5ml fresh culture medium. 4. Centrifuge at 1000rpm (~220g) for 5 minutes under room temperature. 5. Remove the supernatant and resuspend the cells in fresh culture medium. 6. Culture the cell in T75 flask.
Safety Precaution: it is highly recommended that protective gloves and clothing should be used when handling frozen vials.
Standard Culture Procedure 1. Fibroblasts can be cultured in DMED + 10% FBS + 1% P/S. 2. When cells reach ~80-90% confluence, remove the medium, and wash once with sterile PBS (5ml/T75 flask). 3. Add ~2.5ml of 0.25% Trypsin-EDTA to the flask and incubate for ~3 minutes at 37°C. Neutralize the enzyme by adding 2-3 volumes of cell culture medium. 4. Centrifuge 1000rpm (~220g) for 5min and resuspend the cells in desired volume of medium. 5. Seed new culture vessels at 3 × 103 cells/cm2. |
[1] Genetics Home Reference. U.S. National Library of Medicine. 2014-05-05. Retrieved 2014-05-10. [2] De Vlieghere E, et al and De Wever O. Cancer-associated fibroblasts as target and tool in cancer therapeutic and diagnostics. Virchow Arch. 2015.
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